These will consist of lectures, laboratory demonstrations, laboratory work (including independent investigation to identify unknown organisms), reading assigned scientific papers, writing a final laboratory report and answering those questions that are asked in lecture and laboratory. The unknown number 123 handed out by the professor on march 20, 2014 contained both a gram positive bacteria and a gram negative bacteria at this point everything that had been learned in microbiology lab and that had been explained in our lab manual (1) was put into action. The purpose of this experiment was to learn how to isolate and identify unknown bacteria using methods from the lab methods that were used during this experiment included gram staining, streaking for isolation using the quadrant streak method, and differential tests critical tests used in this.
The first method used to identify the unknown bacteria was an isolation streak plate, which utilized four streaks of the unknown mixture onto a nutrient agar plate, via inoculating loop this procedure was used in order to attempt to isolate separate pure colonies from the unknown mixture. Welcome anti essays offers essay examples to help students with their essay writing our collection includes thousands of sample research papers so you can find almost any essay you want. For identification of unknown microbial organisms, dna sequencing rapidly provides data that are more accurate, robust and reproducible than relying solely on visual phenotypic characteristics this is because the sequence-based result is not dependent on age and health of an organism, growth conditions or ancillary testing.
Introduction microorganisms play an highly of import function in dirt ecology soil bacteria interrupt down organic affair into simpler compounds ( clark ) bacteria in the dirt drama of import functions in different biochemical rhythms such as the c rhythm ( clark ). The organism identification tests are performed following bioburden, environmental monitoring, tissue testing, microbial limits, and on sterility or bi positives test systems available are: microseq® library and an applied biosystems/hitachi 3500xl genetic analyzer. Microbiology and unknown bacteria lab unknown bacteria lab report introduction the purpose to this lab was to identify an unknown bacteria from a mixed culture provided to us by our instructor this study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class.
This vial contained two unknown bacteria, one gram negative and one gram positive, that required identification the process of identification was achieved by utilizing procedures learned during the present semester. Microbiology lab essay sample the primary focus of this lab was on microscopy and simple stains crystal violet and carbol fuchsine, simple staining components, were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. First, the bacteria mix inside the unknown 109 tube was streaked across a nutrient agar plate using the quadrant streak technique the quadrant streak helped spread out the bacteria and made it easier for the two different bacteria to make colonies that could be differentiated by the naked eye. Method get downing from an original one gm sample of agricultural dirt a 10-2 dilution was created and used to make a tryptic soy agar ( tsa ) run home base ( robertson and egger 2010 ). Identification of unknown plasmid this research paper identification of unknown plasmid and other 64,000+ term papers, college essay examples and free essays are available now on reviewessayscom autor: review • november 30, 2010 • research paper • 3,534 words (15 pages) • 1,412 views.
The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology it is suggested that culture 11 is a sample of enterobacter aerogenes. Essay on identifying an unknown microbial organism - identifying an unknown microbial organism introduction the purpose of identifying an unknown microbial agent is so that the five i's of microbiology, which are, inoculation, incubation, isolation, inspection and identification can be practiced. Identifying an unknown organism essay unknown microorganisms may 1, 2013 this paper is based on an experiment done in the laboratory over a period of time using the standard method of identifying an unknown microorganism relying on features such as morphology, arrangements and biochemical reactions - identifying an unknown organism essay introduction. Since the unknown bacteria was a gram negative rod, a gelatin test was performed and the organism was inoculated into a gelatin agar tube and placed into the incubator if protein was broken down by gelatinase the tube appeared to have white substances in the tube. The identification of unknown cultures is a practical application of a classification scheme, so that a new isolate can be recognized as a member of an existing species.
Principle: identification of unknown bacteria is one of the major responsibilities of the microbiologists samples of blood, tissue, food, water and cosmetics are examined daily in laboratories throughout the world for the presence of contaminating microorganisms. Identifying the unknown bacterium was determined by separating and differentiating possible bacteria based on specific biochemical characteristics the differential tests used to identify the unknown cultures were gram staining, oxidase, indole test, urea test, and casein test. 1 identification of two unknown species of bacteria by anna albrecht (microbiology 1420) introduction axonomy is defined as ―the science that studies organisms in order to arrange them into.
The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture it produces benefits for many aspects of the research of microorganisms and helps physicians correctly treat patients. A very important technique in microbiology laboratories is identifying unknown bacteria cultures unknown identification is used for practical purposes, such as diagnosing diseases or determining treatment of an infection. This test is used to identify bacteria capable of hydrolyzing urea using the enzyme urease it is commonly used to distinguish the genus proteus from other enteric bacteria the hydrolysis of urea forms the weak base, ammonia, as one of its products. Identification of proteus vulgaris from an unknown sample praise selah g dagoc mambajao, camiguin abstract identification of microorganisms from unknown sample is a routine work for a registered medical technologist assigned in the microbiology section of the laboratory.